IL-10 Mouse anti-Human, Biotin, Clone: 945A 5A10, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen AHC7109

Catalog No. AHC7109

Description

This product is recommended as a detection antibody in Sandwich ELISA applications.

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

Specifications

• Antigen: IL-10
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Gene: IL10
• Gene Alias: CSIF, IL10A, MGC126450, MGC126451, TGIF, Interleukin-10
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3586
• Content And Storage: 4° C
• Form: Liquid

• Applications: ELISA
• Clone: 945A 5A10
• Conjugate: Biotin
• Gene Accession No.: P22301
• Gene Symbols: IL10
• Immunogen: Recombit human IL-10.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ