IL-4 Rat anti-Mouse, Brilliant Violet 650, Clone: 11B11, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 564004
The 11B11 monoclonal antibody specifically binds to mouse Interleukin-4 (IL-4). The immunogen used to generate the 11B11 hybridoma was partially purified mouse IL-4 prepared from the supernatant of Phorbol 12-Myristate 13-Acetate (PMA)-stimulated EL-4 cells. The 11B11 antibody is reportedly a neutralizing antibody.
The antibody was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|IL4: Interleukin-4; BSF-1; B-cell growth factor 1; BCGF-1|
|Partially Purified Mouse IL-4|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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