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IL-6 Rabbit anti-Human, Mouse, Rat, Monkey, HRP, Polyclonal, Novus Biologicals™

Rabbit Polyclonal Antibody

Manufacturer:  Novus Biologicals NBP142762

Catalog No. NBP142762


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Description

Description

IL-6 Polyclonal antibody specifically detects IL-6 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin).
Specifications

Specifications

IL-6
LYOPH
Western Blot 1:1000-1:5000, ELISA 1:10000-1:50000, Immunohistochemistry 1:500-1:2500, Immunohistochemistry-Paraffin 1:500-1:2500
Affinity Purified
IL6
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
24 kDa
0.1 mg
Adaptive Immunity, Cancer, Cell Cycle and Replication, Cytokine Research, Immunology, Innate Immunity
Primary
3569
Reconstitute with 100 ul deionized water.
ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
HRP
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Gentamicin Sulfate
B cell stimulatory factor-2, B-cell differentiation factor, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFN-beta-2, IL-6B-cell stimulatory factor 2, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6
Rabbit
Serum
Immunogen affinity purified
RUO
Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
Polyclonal
This antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
Human, Monkey, Mouse, Rat
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