IFN gamma Goat anti-Mouse, Rat, Polyclonal, Invitrogen
Goat Polyclonal Antibody
Manufacturer: Invitrogen PA124782
PA1-24782 detects Interferon gamma from mouse and rat samples. PA1-24782 will not react with human. PA1-24782 has been successfully used in Western blot, ELISA, neutralization and immunohistochemistry procedures. The Neutralization Dose 50 (ND50) for anti-rat IFNG is approximately 0.08 to 0.2 µg/mL in the presence of 2.5 ng/mL of recombit rat IFNG, measuring the protection of mouse L-929 cells from the lytic effect of the virus. The PA1-24782 immunogen is purified, recombit rat IFNG, expressed in E. coli. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
|Purified, recombit rat IFNG, expressed in E. coli.|
|Antigen affinity chromatography|
|ELISA, Immunohistochemistry, Inhibition Assays, Neutralization, Western Blot|
|PBS with 5% trehalose and no preservative|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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