Involucrin Rabbit anti-Human, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137934
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin tissue.Involucrin is a soluble cytoplasmic protein precursor of the epidermal cornfied envelope that becomes cross-linked by transglutaminase during envelope assembly. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea which lacks a distinct cornfied layer. The protein is a useful marker of terminal differentiation. It is tightly linked to the onset of differentiation and first expressed in the immediate suprabasal layers of the epidermis.
|PBS with 1% BSA and 0.1% sodium azide; pH 7.6|
|Recombit protein encoding aa 420-578 of human involucrin.|
|Antigen affinity chromatography|
|Immunohistochemistry (Paraffin), Western Blot|
|-20° C, Avoid Freeze/Thaw Cycles|
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