Ki-67 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138032
Ki-67 is a nuclear protein, which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-. M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein.This antibody is predicted to react with bovine based on sequence homology.Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil, lymph node or breast carcinoma.
|PBS with 1% BSA and 0.099% sodium azide; pH 7.6|
|Synthetic peptide derived from C-terminus of human Ki-67 protein|
|Antigen affinity chromatography|
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