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Ki-67 Mouse, Brilliant Violet 421, Clone: B56, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 562899

Catalog No. BDB562899

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The B56 monoclonal antibody specifically binds to the Ki-67 antigen that is expressed in the nucleus of cycling cells (G1, S, G2, M cell cycle phases). During the G0 phase, the antigen cannot be detected. During interphase of the cell cycle, it is associated with nucleolar components, and it is on the surface of the chromosomes during M phase. Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear. Because of the strict association of Ki-67 expression with cell proliferation, anti-Ki-67 antibodies are useful for the identification, quantification, and monitoring of growing cell populations.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.



MKI67; Antigen identified by monoclonal antibody Ki-67; KIA
Affinity Purified
Human Ki-67
50 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Brilliant Violet 421
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
IgG1, κ
Human, Murine, Rat, Rhesus Monkey
Product Certifications


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