MilliporeSigma™ anti-Kpnbeta1, Polyclonal

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ST110250UG

Catalog No. ST110250UG

Description

Specifically detects Kpnβ₁ Clone: in Human, Mouse, Xenopus samples, and it is validated for Immunoprecipitation

Full-length Kpnβ₁ (Accession,num; NP_002256) consists of 19 HEAT repeats that form a compact superhelical coil. While other members of the Kpnβ family bind their substrates directly, Kpnβ₁ uses Kpnα as an adaptor for binding substrates that contain a classical NLS. However, Kpnβ₁ can also import some substrates, such as HIV Rev, GAL4, T-cell tyrosine phosphatase, cyclin B1, and parathyroid hormone-related protein (PTHrP), without binding Kpnα. Kpnβ₁ docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importinα enters the nucleoplasm with its passenger protein and importinβ remains at the pore. Interactions between Kpnβ₁ and the FG repeats of nucleoporins are essential in translocation through the pore complex.

Specifications

• Antigen: Kpnβ₁
• Classification: Polyclonal
• Formulation: In Tris-citrate/phosphate buffer, pH 7-8.
• Immunogen: a synthetic peptide corresponding to amino acids near the C-terminus of human Kpnβ₁
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽97kDa Kpnβ₁ protein in Jurkat cells.
• Content And Storage: −20°C
• Isotype: IgG

• Applications: Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Rabbit
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Xenopus species
• Form: Purified

For Research Use Only