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Firefly luciferase Mouse anti-Luciferase, Clone: Luci17, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA112556

Catalog No. 01-674-342


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Description

Description

MA1-12556 detects Luciferase in C. elegans and Drosophilia melanogaster tissue, human fibroblasts, mouse macrophages, kidney, liver and cortex tissues, as well as NIH-3T3, Jurkat and BHR21 cell lines with little to no background signal.

Luciferase is a luminescent enzyme produced by Photinus pyralis, also referred to as the common eastern firefly or North American firefly. It is known for its role as a reporter protein and can be used to quantify expression levels from promoters in tissue culture and in vivo. Luciferase catalyses the oxygenation of luciferin using ATP and oxygen to produce oxyluciferin, a highly unstable, singlet-excited compound that emits light upon relaxation to its ground state. Bioluminescence assay systems have become increasingly used in biology and medical research laboratories in addition to (or as alternatives to) fluorescence and chemiluminescence detection strategies. Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and dual-spectral luciferase reporter assays.
Specifications

Specifications

Firefly Luciferase
Monoclonal
1 mg/mL
PBS with 0.08% sodium azide
Luciferin 4-monooxygenase; Firefly Luciferase; Green Luciferase; Japanese firefly; Genji firefly; Photinus pyralis
Luciferase protein isolated from Photinus pyralis.
Protein A/G
RUO
Antibody
Monoclonal
Immunohistochemistry (Frozen), Western Blot
Luci17
Unconjugated
Liquid
Mouse
IgG1
100 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
Luciferase
Documents
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