CD357 (AITR/GITR) Rat anti-Mouse, Functional Grade, Clone: DTA-1, eBioscience™ 

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 16587481

Catalog No. 50-145-87

Description

Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predomitly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease. Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis and immunohistochemistry of frozen mouse tissue. It has also been reported for use in functional assays. Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This DTA-1 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. Glucocorticoid-induced tumor necrosis factor receptor family-related gene (GITR) is a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR.

Specifications

• Antigen: CD357 (AITR/GITR)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: O35714
• Gene Alias: AITR, GITR-D, TNFRSF18
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
• Clone: DTA-1
• Conjugate: Functional Grade
• Gene: Tnfrsf18
• Format: Liquid
• Gene Symbols: Tnfrsf18
• Isotype: IgG2b, kappa
• Quantity: 50 μg
• Storage Requirements: 4° C
• Primary or Secondary: Primary
• Gene ID (Entrez): 21936

For Research Use Only.