CD90.2 (Thy-1.2) Rat anti-Mouse, FITC, Clone: 30-H12, eBioscience™ 

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 11090382

Catalog No. 50-969-7

Description

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer ft.s cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Specifications

• Antigen: CD90.2 (Thy-1.2)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01831
• Gene Alias: CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2,Thy 1.2; thy-1 antigen; thy-1 membrane glycoprotein
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse

• Applications: Flow Cytometry
• Clone: 30-H12
• Conjugate: FITC
• Gene: Thy1
• Format: Liquid
• Gene Symbols: Thy1
• Isotype: IgG2b, kappa
• Quantity: 100 μg
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Primary or Secondary: Primary
• Gene ID (Entrez): 21838

For Research Use Only.