MHC Class I (H-2Db) Mouse anti-Mouse, Biotin, Clone: 28-14-8, eBioscience™ 

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 13599985

Catalog No. 50-121-28

Description

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: 28-14-8 has been reported for use in flow cytometric analysis. Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

Specifications

• Antigen: MHC Class I (H-2Db)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P14426
• Gene Alias: H2DB, H-2 class I histocompatibility antigen D-B alpha chain, H2D1
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse

• Applications: Flow Cytometry
• Clone: 28-14-8
• Conjugate: Biotin
• Gene: H2-D1
• Format: Liquid
• Gene Symbols: H2-D1
• Isotype: IgG2a, kappa
• Quantity: 500 μg
• Storage Requirements: 4° C, do not freeze
• Primary or Secondary: Primary
• Gene ID (Entrez): 14964

For Research Use Only.