CD90.1 (Thy-1.1) Mouse anti-Mouse, Rat, PE, Clone: HIS51, eBioscience™ 

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12090081

Catalog No. 50-105-05

Description

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells. Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer ft.s cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Specifications

• Antigen: CD90.1 (Thy-1.1)
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01831, P01830
• Gene Alias: THY1, Thy-1 cell surface antigen, FLJ33325, Thy-1 T-cell antigen, Thy-1 membrane glycoprotein
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse, Rat

• Applications: Flow Cytometry
• Clone: HIS51
• Conjugate: PE
• Gene: Thy1
• Format: Liquid
• Gene Symbols: Thy1
• Isotype: IgG2a, kappa
• Quantity: 50 μg
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Primary or Secondary: Primary
• Gene ID (Entrez): 21838, 24832

For Research Use Only.