OVA257-264 (SIINFEKL) peptide bound to H-2Kb Mouse anti-Mouse, PE, Clone: eBio25-D1.16 (25-D1.16), eBioscience™ 

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12574381

Catalog No. 50-108-44

Description

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Cells can be pulsed with the SIINFEKL peptide according to the following protocol: 1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM. 2. Incubate cells at 37°C for 2 hours. 3. Wash cells with flow staining buffer. 4. Proceed with cell surface staining as normal. For additional information see the references listed below. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.

2 µm post-manufacturing filtered.

Specifications

• Antigen: OVA257-264 (SIINFEKL) peptide bound to H-2Kb
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Alias: H-2Kb-SIINFEKL; OVA-Kb; OVA257-264 H-2Kb; OVA257-264
• Isotype: IgG1, kappa
• Quantity: 50 μg
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Primary or Secondary: Primary
• Target Species: Mouse

• Applications: Flow Cytometry
• Clone: eBio25-D1.16 (25-D1.16)
• Conjugate: PE
• Format: Liquid
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal

For Research Use Only.