M-CSF Rat anti-Mouse, Clone: ER-MP58, Invitrogen
Rat Monoclonal Antibody
Manufacturer: Invitrogen MA140079
MA1-40079 detects ER-MP58, an antigen present on all mouse macrophage colony stimulating factor (M-CSF) responsive cells. MA1-40079 has been successfully used in immunohistochemistry (frozen), ELISA, and flow cytometry applications. Typical starting dilution is 1:50. MA1-40079 recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells to the monocytic level, but also on granulocytes. Expression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commonly used myeloid markers as Mac-1, F4/80, and Gr-1.M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
|PBS with 0.1% BSA and 0.02% sodium azide|
|ELISA, Flow Cytometry, Immunohistochemistry (Frozen)|
|Myeloid precursor cells.|
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