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MAL Rabbit anti-Human, Mouse, Rat, Xenopus laevis, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1085

Catalog No. PA1-085

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PA1-085 detects MAL from rat, mouse, xenopus and human samples. PA1-085 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~ 17 kDa protein corresponding to MAL from rat kidney extracts. The PA1-085 immunogen is a synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL. This peptide (Cat. No. PEP-249) can be used in neutralization and control experiments.

MAL, a nonglycosylated integral membrane proteolipid, has been identified as a major internal component of glycolipid-enriched membrane (GEM) microdomains. MAL is the first integral membrane proteolipid component to have been shown as a necessary element in the cellular apical sorting apparatus for newly synthesized proteins. In its steady state this highly hydrophobic proteolipid, resides predomitly in the apical zone of polarized epithelial cells. It has also been shown in itinerant protein cycling between the plasma membrane and endosomes and in other cells. This 17-kDa proteolipid contains four putative transmembrane domains. These domains along with its protein trafficking properties are defined by the specific signals delegated by its specific amino acid sequence.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly



PBS with 1mg/mL BSA and 0.05% sodium azide
P21145, O09198, Q64349
100 μg
-20° C, Avoid Freeze/Thaw Cycles
17153, 25263, 379738, 4118
Western Blot
MAL, mal.L
Synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL.
Antigen affinity chromatography
Human, Mouse, Rat, Xenopus laevis
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