MARVELD2 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen 488400
This antibody is specific for the Tricellulin/MARVELD-2 protein. On western blots, it identifies the target band at ~64 kDa. Reactivity has been confirmed with human Caco-2, dog MDCK, mouse IMCD-3 cells, and mouse kidney lysates by western blotting, and with mouse MTE7b cells by immunocytochemistry. Based on amino acid sequence homology, reactivity with Rhesus monkey, chimpanzee, rat, bovine, and horse is expected.Tight junctions form an important barrier of paracellular transport in epithelial cells. Sealing of two adjacent cells at bicellular tight junctions (bTJ), is well described. The main structural components of bicellular tight junctions are claudins and occludin, all tetra membrane-spanning proteins. Less is known about the structure of tricellular tight junctions (tTJ), a point where three adjacent cells are in contact with each other. Tricellulin/MARVELD-2 (MARVEL domain-containing protein 2) is the first protein identified that specifically concentrates in tricellular tight junctions. This protein has four membrane-spanning domains, similar to claudins. Tricellulin expression is high in epithelium-derived tissues, such as small intestine, kidney, and lung. Functional evidence for the role of tricellulin in tight junction formation comes from siRNA studies, where suppression of its expression leads to compromised epithelial barrier and tight junction formation. Mutations in tricellulin can cause nonsyndromic deafness (DFNB49). These loss-of-function mutations remove all or most of a conserved region in the cytosolic domain that binds to the cytosolic scaffolding protein ZO-1, indicating that interaction with other known tight junction proteins plays an important role for the function of tricellulin. An independent study in Pakistani families confirmed the role of tricellulin mutations for hearing impairment.
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|Recombit protein derived from the C-terminal region of the human Tricellulin protein (AccessionNo. NP_001033692, Q8N4S9), which is identical to rhesus monkey and chimpanzee sequences, and 89% homologous to rat and mouse, 88% homologous to bovine and canine, and 86% homologous to horse sequence|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 0.1% sodium azide; pH 7.4|
|DFNB49, MARVD2, MRVLDC2, Tric, MARVEL (membrane-associating) domain containing 2, MARVEL domain-containing protein 2, tricellulin|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|153562, 218518, 365657|
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