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MBD1 Mouse anti-Human, Mouse, Clone: 100B272.1, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA141025

Catalog No. 01-676-441


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Description

Description

Suggested positive control: Hela whole cell extract, HeLa cell lysate.

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).
Specifications

Specifications

MBD1
Monoclonal
0.5 mg/mL
PBS with 0.05% BSA and 0.05% sodium azide
Q9UIS9, Q9Z2E2
CXXC3, PCM1, RFT
Mouse
IgG1
100 μg
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
17190, 4152
ChIP assay, Immunoprecipitation, Western Blot
100B272.1
Unconjugated
MBD1
Liquid
MBD1
Synthetic peptide corresponding to human MBD1.
Protein G
RUO
Antibody
Monoclonal
Human, Mouse
Documents
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