MCM2 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138099
This antibody is predicted to react with bovine, canine, porcine and rabbit based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.The mini-chromosome maintece (MCM) family of proteins, including MCM2, MCM3, MCM4(Cdc21), MCM5(Cdc46), MCM6(Mis5) and MCM7(Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro.
|Synthetic peptide corresponding to N-terminus of human MCM2 protein.|
|Antigen affinity chromatography|
|Immunohistochemistry (Paraffin), Western Blot|
|PBS with 1% BSA and 0.1% sodium azide; pH 7.6|
|BM28, CCNL1, CDCL1, D3S3194, MITOTIN, cdc19, DNA replication licensing factor MCM2, MCM2 minichromosome maintece deficient 2, mitotin, cell devision cycle-like 1, cyclin-like 1, minichromosome maintece deficient 2 mitotin, minichromosome maintece protein 2 homolog, nuclear protein BM28|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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