HLA-ABC Mouse anti-Bovine, Human, Non-human primate, Rodent, Clone: W6/32, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA180123
This antibody does not react with amphibia, chicken, dog, goat, guinea pig, mouse or rabbit. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug. Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determit shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains.HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
|PBS with no preservative|
|P01889, P10321, P05534|
|MHC Class 1, MHC class I, MHC I HLA, HLA-A, HLA-B, HLA-C, MHC HLA-ABC, HLA-B 1502|
|-20° C, Avoid Freeze/Thaw Cycles|
|3105, 3106, 3107|
|ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation|
|HLA-A, HLA-B, HLA-C|
|Purified human tonsil lymphocyte membranes.|
|Bovine, Human, Non-human Primate, Rodent|
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