MilliporeSigma™ anti-MMP-9 Clone: 6-6B,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ IM09L100UG

Catalog No. IM09L100UG

Description

Specifically detects MMP-9 Clone: 6-6B in Human samples, and it is validated for Immunoprecipitation, Neutralization

Matrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP's share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ∽10kDa segment from the N terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-9 (Gelatinase B, 92kDa gelatinase/type IV collagenase) is secreted as a 92kDa zymogen which is proteolytically processed to the 83kDa active form; a 68kDa has also been detected. MMP-9, along with its most closely related member of the MMP family, MMP-2, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential. Elevated levels of MMP-9 in plasma suggest that it may be a useful marker for the diagnosis or prognosis of cancer in general.

Specifications

• Antigen: MMP-9
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

• Applications: Immunoprecipitation, Neutralization
• Clone: 6-6B
• Formulation: Lyophilized from a volatile buffer, 100μg BSA.
• Immunogen: MMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽92kDa latent and the ∽83kDa active forms of MMP-9 under non-reducing conditions, but only the latent form under reducing conditions. Inhibits the enzymatic activity of MMP-9.
• Content And Storage: 2°C to 8°C
• Isotype: IgG1

For Research Use Only