MMP14 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138193
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta or breast carcinoma.MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of the matrix metalloproteinase family, ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively, contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT1-MMP gets activated through the membrane plasmin cascade. It binds to TIMP-2 first and then to MMP-2 forming a trimer. It cleaves the pro-MMP-2 to its active form.
|PBS with 1% BSA and <0.1% sodium azide; pH 7.6|
|1, MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, matrix metalloproteinase 14 membrane-inserted, matrix metalloproteinase-14, membrane type 1 metalloprotease, membrane-type matrix metalloproteinase 1, membrane-type-1 matrix metalloproteinase|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)|
|Synthetic peptide derived from internal region of human MMP-14 protein.|
|Antigen affinity chromatography|
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