MMP15 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138195
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT2-MMP plays a minor role in activation of pro-MMP-2 to its active form in breast carcinomas. Coexpression of MT1-MMP and MT2-MMp in advanced stages of breast carcinomas is indicative of possible involvement of MT2-MMP in its metastasis.
|PBS with 1% BSA and 0.1% sodium azide; pH 7.6|
|MT2-MMP, MTMMP2, SMCP-2, MMP-15, MT-MMP 2, MT2MMP, matrix metalloproteinase 15 membrane-inserted, matrix metalloproteinase-15, membrane-type matrix metalloproteinase 2, membrane-type-2 matrix metalloproteinase|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|Synthetic peptide from the catalytic domain of mouse MMP-15 / MT2-MMP protein|
|Antigen affinity chromatography|
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