anti-CD45RA, DyLight 550, Clone: 158-4D3, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP233144R
DescriptionCD45RA Monoclonal antibody specifically detects CD45RA in Human, Primate samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
|Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500|
|Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.|
|Protein G purified|
|Store at 4C in the dark.|
|Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).|
|Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)|
|50mM Sodium Borate with 0.05% Sodium Azide|
|B220, CD45 antigen, CD45R, EC 18.104.22.168, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide|
|Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers|
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