anti-Galectin-3, Clone: B2C10, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP142384
DescriptionGalectin-3 Monoclonal antibody specifically detects Galectin-3 in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Functional, Immunoassay.
|PBS, containing 0.1% bovine serum albumin. with No Preservative|
|The antibody reacts with the Galectin-3 antigen.|
|Protein A or G purified|
|Store at 4C. Do not freeze.|
|The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3's hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.|
|Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot|
|Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Functional, Immunoassay|
|Carbohydrate-binding protein 35, CBP 35,35 kDa lectin, Gal-3, galactin-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBPCBP35, galectin-3, GALIG, IgE-binding protein, L31, L-31, Laminin-binding protein, Lectin L-29, lectin, galactoside-binding, soluble, 3, LGALS2, MAC-2, Mac-2 antigen, MAC2GAL3|
|Apoptosis, Cancer, Cellular Markers, Tumor Suppressors|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only