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anti-MUC-1, Clone: VU-12E1, Novus Biologicals™

Manufacturer:  Novus Biologicals NBP142160

Catalog No. NBP142160

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Specifications

Specifications

MUC-1
VU-12E1
Unconjugated
0.1 mg/ml PBS with 0.05% Sodium Azide
Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, CD227, CD227 antigen, DF3 antigen, EMA, episialin, H23 antigen, H23AG, KL-6, MAM6, MUC-1, MUC1/ZD, mucin 1, cell surface associated, mucin 1, transmembrane, mucin-1, Peanut-reactive urinary mucin, PEMMUC-1/SEC, PEMT, Polymorphic epithelial mucin, PUMMUC-1/X, tumor associated epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Mouse
IgG1
0.1 mg
Cancer, Cellular Markers, Extracellular Matrix
Primary
4582
Human
ELISA, Immunohistochemistry, Immunohistochemistry (Frozen)
0.1 mg/mL
ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Purified
MUC1
The antibody reacts with the MUC1 antigen.
Protein A or G purified
RUO
Store at 4C. Do not freeze.
Monoclonal
VU-12E1 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largly consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of mAb VU12E1 is the 5-mer PDTRP of the MUC1 tandem repeat as established with “epitope fingerprinting”. Source: A Balb/c mouse was immunized with live cells (107 cells/dose in PBS) of human breast cancer cell line ZR-75-1. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by subcloning four times.
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