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anti-MyoD, Biotin, Clone: SPM427, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP234772B

Catalog No. NBP234772B


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Description

Description

MyoD Monoclonal antibody specifically detects MyoD in Human, Mouse, Rat, Chicken samples. It is validated for Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
Specifications

Specifications

MyoD
SPM427
Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Purified
MYOD1
Recombinant mouse MyoD1 protein
45 kDa
0.1 ml
Cancer, Cell Cycle and Replication, Growth and Development
Primary
4654
Chicken, Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Biotin
PBS with 0.05% Sodium Azide
bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
Mouse
IgG1 Kappa
Protein A purified
RUO
Store at 4C in the dark.
Monoclonal
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
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