Myogenin Antibody (MGN185), Alexa Fluor™ 405, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP233056AF405

Catalog No. NBP233056W

Description

Myogenin Monoclonal specifically detects Myogenin in Human, Mouse, Rat, Porcine, Feline samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Myogenin
• Classification: Monoclonal
• Conjugate: Alexa Fluor 405
• Gene Alias: BHLHC3, bHLHc3Myogenic factor-4; myogenin, Class C basic helix-loop-helix protein 3, myf-4, MYF4myogenin, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)
• Host Species: Mouse
• Molecular Weight of Antigen: 34 kDa
• Quantity: 0.1 mL
• Research Discipline: Cancer, Stem Cell Markers, Transcription Factors and Regulators
• Gene ID (Entrez): 4656
• Target Species: Human, Mouse, Rat, Porcine, Feline
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: MGN185
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Gene Symbols: MYOG
• Immunogen: Human Myogenin recombinant protein (Uniprot: P15173)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

For Research Use Only