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anti-Myogenin, Alexa Fluor 405, Clone: MGN185, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP233056AF405

Catalog No. NBP233056W


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Description

Description

Myogenin Monoclonal antibody specifically detects Myogenin in Human, Mouse, Rat, Porcine, Feline samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
Specifications

Specifications

Myogenin
MGN185
Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Purified
MYOG
Human myogenin recombinant protein
34 kDa
0.1 ml
Cancer, Stem Cell Markers, Transcription Factors and Regulators
Primary
4656
Human, Mouse, Rat, Porcine, Feline
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Alexa Fluor 405
50mM Sodium Borate with 0.05% Sodium Azide
BHLHC3, bHLHc3Myogenic factor-4; myogenin, Class C basic helix-loop-helix protein 3, myf-4, MYF4myogenin, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)
Mouse
IgG1
Protein G purified
RUO
Store at 4C in the dark.
Monoclonal
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
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