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anti-TAG-72, DyLight 650, Clone: B72.3, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP233180C
DescriptionTAG-72 Monoclonal antibody specifically detects TAG-72 in Human, Rat, Bovine, Canine, Hamster samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
|Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500|
|Store at 4C in the dark.|
|Recognizes an oncofetal antigen of >1,000kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.|
|Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)|
|50mM Sodium Borate with 0.05% Sodium Azide|
|CA 72-4, Protein TAG-72, tag 72, tag-72 Protein TAG-72|
|A membrane enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for the TAG-72 (B72.3) antibody.|
|Protein G purified|
|Human, Rat, Bovine, Canine, Hamster|
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