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NEFM Mouse anti-Human, Mouse, Rat, Clone: RMO-270, Invitrogen™ Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 130700

Catalog No. 13-070-0


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Description

Description

The cloning partner for this antibody is Sp/2. This antibody reacts with the 160 kD polypeptide subunit of human neurofilament. It specifically recognizes a phosphate-independent epitope in the tail (carboxy) domain of NF-M of most vertebrates and invertebrates. This antibody is suitable for immunohistochemical staining of Bouin ft.s and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

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Specifications

Specifications

NEFM
Monoclonal
0.5 mg/mL
PBS with 0.1% sodium azide
P07197, P08553, P12839
NEF3, NF-M, NFM, 160 kDa neurofilament protein, neurofilament 3, neurofilament medium polypeptide, neurofilament triplet M protein, neurofilament, medium polypeptide 150kDa, neurofilament-3 (150 kD medium)
Mouse
IgG2a, kappa
200 μg
-20°C
Primary
18040, 24588, 4741
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
RMO-270
Unconjugated
NEFM
Liquid
NEFM
Rat neurofilaments
Protein A
RUO
Antibody
Monoclonal
Human, Mouse, Rat
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