Ly-6G/Ly-6C Rat anti-Mouse, Clone: NIMP-R14, Invitrogen
Rat Monoclonal Antibody
Manufacturer: Invitrogen MA140038
This clone is highly specific for murine Ly-6G and Ly-6C. It has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with this antibody in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. The endotoxin concentration is < 24 EU/mg, determined with LAL Assay. Suggested positive control: Mouse neutrophils; Suggested negative control: Mouse thymocytesThe Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predomitly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear.
|PBS with 0.1% BSA and no preservative|
|P0CW02, P0CW03, P35461|
|Ly6c1, Ly6c2, Ly6g|
|Purified BALB/c mouse neutrophils.|
|ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)|
|100041546, 17067, 546644|
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