NFAT5 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1023
PA1-023 detects nuclear factor of activated T-cells 5 (NFAT5) from human and mouse cells as well as recombit human protein. PA1-023 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, gel shift and immunocytochemistry procedures. By Western blot, this antibody detects an ~170 kDa protein representing NFAT5 in HEK293 cells transfected with the human NFAT5 gene. Immunocytochemical staining of NFAT5 in HEK293 cells transfected with the human NFAT5 gene with PA1-023 results in primarily cytoplasmic staining. The PA1-023 immunogen is a synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. This sequence is 94% conserved in the mouse protein. PA1-023 immunizing peptide (Cat. No. PEP-123) is available for use in neutralization and control experiments.The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
|Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.|
|Antigen affinity chromatography|
|Gel Shift, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Nuclear Factor Activated T-Cells, NFATL1, Nuclear factor of activated T cells 5, T cell transcription factor NFAT5, NF-AT5, Tonicity-responsive enhanc|
|-20° C, Avoid Freeze/Thaw Cycles|
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