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NFkB p50 Rabbit anti-Human, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA138278

Catalog No. 01-673-937


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Description

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil, testis or prostate tissue.

NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor. Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex. The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.
Specifications

Specifications

NFkB p50
Polyclonal
PBS with 1% BSA and <0.1% sodium azide; pH 7.4
P19838
EBP-1; KBF1; NF-kB1; NF-kappa-B; NF-kappaB; NFKB-p105; NFKB-p50; NFkappaB; p105; p50; DNA binding factor KBF1; DNA-binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit; nf?b
Rabbit
IgG
1 mL
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
4790
Immunohistochemistry (Paraffin)
Unconjugated
NFKB1
Liquid
NFKB1
Synthetic peptide derived from N-terminus of human NFkB p50
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human
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