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eNOS Rabbit anti-Bovine, Canine, Human, Mouse, Porcine, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1037

Catalog No. PA1037


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Description

Description

PA1-037 detects endothelial nitric oxide synthase (eNOS) from bovine, canine, human, mouse, porcine and rat tissues as well as recombit protein. This antibody does not detect human iNOS or rat nNOS. PA1-037 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. By Western blot PA1-037 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in human vascular endothelial cells with PA1-037 results in prominent staining of the epithelial cells. The PA1-037 immunogen is a synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

Specifications

eNOS
Polyclonal
Unconjugated
NOS3
Liquid
NOS3
Synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Bovine, Canine, Human, Mouse, Porcine, Rat
Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
0.2 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
P29473, Q9TUX8, P29474, P70313, Q28969, Q62600
Endothelial Nitric Oxide Synthase, NOS Type III, e-NOS
Rabbit
IgG
100 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
18127, 24600, 287024, 397557, 403784, 4846
Documents
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