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NSF Mouse, Unlabeled, Clone: 7, BD

Mouse Monoclonal Antibody



Antigen NSF
Isotype IgG2b
Immunogen Human NSF aa. 488-601
Formulation Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Conjugate Unconjugated
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BD Biosciences
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BD Biosciences
150μg This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A


Eukaryotic protein trafficking involves the packaging of target molecules into membraneous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Components of both the vesicle and the plasma membrane interact to form a fusion complex, which mediates specific docking and fusion of vesicles. This complex contains NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs). SNAP-25 (synaptosome-associated protein of 25kDa) and syntaxin are SNAREs that are associated with the target plasma membrane (t-SNAREs), while synaptobrevin and synaptotagmin are vesicle-associated SNARE proteins (v-SNAREs). SNAREs form a 7S complex that has a high affinity binding site for α-SNAP and NSF. Upon binding of α-SNAP and NSF, a 20S complex is formed that is rapidly disassembled due to NSF's ATPase activity. This facilitates vesicle fusion to the target membrane followed by fusion and release of vesicle components.

Immunofluorescence, Western Blotting



Human NSF aa. 488-601
Cell Biology
Affinity Purified
Store undiluted at -20°C.
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
In vitro Assay, Immunofluorescence, Western Blot
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For Research Use Only.

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