Occludin, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA139441
Occludin is a 65kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. This phosphorylation is thought to be involved in regulating both the localization and the function of Occludin. Polyunsaturated fatty acids are known to up-regulate Occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution.Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is lung tissue.
|TBS with 1% BSA and <0.1% sodium azide; pH 7.6|
|Synthetic peptide corresponding to C-terminus of human occludin|
|Antigen affinity chromatography|
|4° C, do not freeze|
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