OxPhos Rodent WB Mouse anti-Bovine, Human, Mouse, Non-human primate, Rat, Invitrogen
Manufacturer: Invitrogen 458099
This product is a cocktail of multiple OxPhos antibodies. C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I Purification Method: Antigen Affinity Chromatography C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II Purification Method: IgG Fraction C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III Purification Method: IgG Fraction C-IV-I (clone) 1D6E1A8 - Human Complex IV subunit I Purification Method: Ammonium Sulfate Precipitation C-V-alpha (clone) 15H4C4 - Bovine Complex V Purification Method: Antigen Affinity Chromatography The kit is suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from mouse, rat, human, or bovine sources. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used. Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
|OxPhos Rodent WB|
|HEPES buffered saline with 0.02% sodium azide|
|Oxidative Phosphorylation; OxPhos Complex|
|4°C or -80°C if preferred|
|Bovine, Human, Mouse, Non-human primate, Rat|
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok