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OxPhos WB Mouse anti-Human, Invitrogen
Manufacturer: Invitrogen 458199
This product is a cocktail of multiple OxPhos Mouse anti Human antibodies. C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I This is an optimized premixed cocktail suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from human sources. Human heart mitochondria (50 µg) is also included as a Western Blotting control. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used. C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III C-IV-II (clone) 12C4F12 -Human Complex IV, subunit II C-V-alpha (clone) 15H4C4 -Bovine Complex VAltered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
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|OxPhos Human WB|
|HEPES buffered saline with 0.02% sodium azide|
|Oxidative Phosphorylation; OxPhos Complex|
|4° C, do not freeze|
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