p190-B Mouse, Unlabeled, Clone: 54, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611613

Catalog No. BDB611613

Description

GTPase activating proteins (GAPs) stimulate the GTP-hydrolyzing activity of GTPases, such as p21ras and Rho. p190-A is a Ras-GAP associated protein that is tyrosine phosphorylated in transformed and growth factor-stimulated cells. Ras-GAP and p190-A are targets of oncoproteins and growth factor receptors. p190-B is another Ras-GAP in the p190 family. It has 51% identity with p190-A and contains several GTPase-related domains in the N-terminal region and a Rho-GAP domain in the C-terminal region. p190-B is expressed in kidney, brain, liver, and lung, as well as in human foreskin fibroblasts, RD muscle cells, and HT-1080 cells. In fibroblasts, p190-B is localized diffusely in the cytoplasm and co-localizes with the α5β1 integrin receptor for fibronectin. Adhesion of fibronectin-coated latex beads to cells leads to the recruitment of p190-B and Rho to the plasma membrane at sites of bead contact. In addition, the recombinant Rho-GAP domain of p190-B displays GAP activity for RhoA, Rac1, and G25K/CDC42Hs. Thus, p190-B is thought to act as a transmembrane link between integrins and Rho GTPases during fibronectin-induced changes in cell morphology and motility.

Host Species: Mouse
Clone: 54
Isotype: IgG1
Species Reactivity [for Features Main]: Human
Immunogen: Human p190-B aa. 1102-1214

Immunofluorescence, Western Blotting

Specifications

• Antigen: p190-B
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human p190-B aa. 1102-1214
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Human, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 54
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.