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MilliporeSigma™ anti-p21WAF1 Clone: 22,

Mouse Monoclonal Antibody

Manufacturer:  MilliporeSigma™ OP79100UG

Catalog No. OP79100UG

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Specifically detects p21WAF1 Clone: 22 in Human, Mouse, Rat samples, and it is validated for Immunoprecipitation

The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ∽21kDa product of the WAF1 gene is found in a complex involving cyclins, cyclin dependent kinases (CDK), and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is to prevent CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. However, the role of p21WAF1 in apoptosis is less clear although p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 in response to DNA damage can occur by both p53-dependent and p53-independent mechanisms, in response to mitogenic stimuli, differentiation, or in tumor cells with mutated p53. Functional p21WAF1 is essential for p53-mediated G1 arrest presumably due to WAF1 inhibition of both CDK activity and PCNA-dependent DNA replication. WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Not surprisingly, p21WAF1 overexpression is growth suppressive consistent with its role as an inhibitor of CDKs. By inhibiting Rb inactivation in a p53-dependent fashion, p21WAF1 serves to integrate cell cycle control mediated by p53 and Rb.
Specifications

Specifications

p21WAF1
22
In 0.05M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Murine
IgG1
RUO
Primary
Recognizes the ∽21kDa p21WAF1 protein in rat and mouse embryo fibroblast cell lines treated with actinomycin D.
Immunoprecipitation
Unconjugated
Purified
recombinant, mouse p21WAF1
100μg
2°C to 8°C
Monoclonal
Human, Murine, Rat
SDS
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