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p53 Mouse anti-Human, Rhesus Monkey, Clone: PAb 240, Invitrogen™ Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen AHO0112

Catalog No. AHO0112


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Description

Description

This antibody reacts with only mutant p53 protein under non-denaturing conditions (Immunoprecipitation) but reacts with both mutant and wild p53 under denaturing conditions (Western blot). It reacts with an epitope at aa 212-217. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls include MDAD-231 cells, SK-BR-3 cells, colon carcinoma, or squamous cell carcinoma.

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and maligcy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human maligcies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

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Specifications

Specifications

p53
Monoclonal
0.2 mg/mL
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
P04637, P56424
antigen NY-CO-13, cellular tumor antigen p53, p53 tumor suppressor, phosphoprotein p53, transformation-related protein 53, TP53
Mouse
IgG1
500μL
4° C
Primary
7157, 716170
Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Pab 240
Unconjugated
TP53
Liquid
TP53
Murine p53-beta-galactosidase fusion protein containing p53 sequence from amino acid14-289.
Protein G
RUO
Antibody
Monoclonal
Human, Non-human Primate, Porcine, Rat, Rhesus Monkey
SDS
Documents
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