p53 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA527822
PA5-27822 targets p53 in IF, IHC (P), IP, ChIP and WB applications and shows reactivity with Human samples. The PA5-27822 immunogen is recombit fragment corresponding to a region within amino acids 142 and 393 of Human p53. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and maligcy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human maligcies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.
|Recombit fragment corresponding to a region within amino acids 142 and 393 of Human p53|
|Antigen affinity chromatography|
|ChIP assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|PBS with 20% glycerol and 0.01% thimerosal; pH 7|
|antigen NY-CO-13, cellular tumor antigen p53, p53 tumor suppressor, phosphoprotein p53, transformation-related protein 53, TP53|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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