DJ-1 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137560
This antibody is predicted to react with bovine, chicken, canine, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.DJ-1 protein has 189 amino acids and was cloned from a HeLa cell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. The expression of DJ-1 is induced by growth signals and is translocated from the cytoplasm to the nucleus during the S phase, although no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway.
|PBS with 1% BSA and <0.1% sodium azide; pH 7.6|
|DJ-1, DJ1, Parkinson disease autosomal recessive, early onset 7, oncogene DJ1, protein DJ-1|
|4° C, do not freeze|
|Immunohistochemistry (Paraffin), Western Blot|
|Synthetic peptide derived from C-terminus of human DJ-1 protein.|
|Antigen affinity chromatography|
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