Parkin Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1751
PA1-751 detects recombit human parkin. PA1-751 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~52 kDa protein representing recombit human parkin. The PA1-751 immunizing peptide corresponds to amino acid residues 298-313 from human parkin. This sequence is completely conserved between human, mouse and rat. PA1-751 immunizing peptide (Cat. No. PEP-159) is available for use in neutralization and control experiments.Parkinson’s Disease, the second most common neurodegenerative disease after Alzheimer’s Disease, is characterized by the loss of dopaminergic neurons and the presence of Lewy bodies (comprised of alpha-synuclein and parkin inclusions). Autosomal Recessive Juvenile Parkinsonism (AR-JP) is a recently described form of Parkinson’s Disease that has been linked to a gene that codes for parkin. Parkin, a 52 kDa protein, has a suggested role in the ubiquitin/proteasome pathway for protein degradation. The amino terminus bears sequence homology to ubiquitin while functionally it acts as a RING-type ubiquitin protein ligase (E3) that coordinates the transfer of ubiquitin to substrate proteins, thus targeting them for degradation by the proteasome. Studies show that parkin interacts with and ubiquitinates the alpha-synuclein interacting protein, synphilin-1. Recent analysis of parkin mutations in AR-JP patients reveals that parkin becomes functionally inactive as an E3 enzyme. This suggests that the loss of function mutation within parkin results in a failure of ubiquitin/proteasome-mediated proteolysis of synphilin-1 and the accumulation of proteins within the cell resulting in neuronal cell death.
|Synthetic Peptide: C I(298) K E L H H F R I L G E E Q Y N(313)|
|Antigen affinity chromatography|
|Immunohistochemistry, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Ubiquitin E3 ligase PRKN, Parkinson juvenile disease protein 2, Parkinson disease protein 2, Prkn, Park2|
|-20° C, Avoid Freeze/Thaw Cycles|
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