PARP1 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138414
This antibody is predicted to react with bovine, chicken, amphibian, mouse and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.PARP uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death and is mediated by the caspase CPP32 protein.
|PBS with 1% BSA and <0.1% sodium azide; pH 7.4|
|ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, ADP-ribosyltransferase NAD+, poly ADP-ribose polymerase, ADP-ribosyltransferase NAD+, ADP-ribosyltransferase diphtheria toxin-like 1, NAD+ ADP-ribosyltransferase 1, poly ADP-ribose polymerase family, member 1, poly [ADP-ribose] polymerase 1, polyADP-|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunohistochemistry (Paraffin), Western Blot|
|Synthetic peptide derived from the N-terminus of human PARP protein|
|Antigen affinity chromatography|
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