PCSK2 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1058
PA1-058 detects prohormone convertase 2 (PC2) from human and mouse alpha pancreas cells. PA1-058 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~65 kDa protein representing PC2 from alpha TC 6 cell extract. This antibody is not recommended for Neuro-2a cells in immunfluorescent applications. The PA1-058 immunogen is a synthetic peptide corresponding to residues E(622) L D E A V E R S L Q S I L R K N(638) of mouse PC2. This sequence is completely conserved between mouse and rat protein. PA1-058 synthetic peptide (PEP-138) is available for use in neutralization and control experiments.The subtilisin-like prohormone convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
|Synthetic peptide corresponding to residues E(622) L D E A V E R S L Q S I L R K N(638) of mouse PC2.|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Prohormone Convertase 2, Neuroendocrine convertase 2, EC 184.108.40.206, NEC 2, Proprotein convertase 2, KEX2-like endoprotease 2|
|-20° C, Avoid Freeze/Thaw Cycles|
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