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PCNA Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA527214

Catalog No. PIPA527214


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Description

Description

PA5-27214 targets PCNA in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples. The PA5-27214 immunogen is recombit fragment corresponding to a region within amino acids 1 and 253 of Human PCNA. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
Specifications

Specifications

PCNA
Polyclonal
Unconjugated
PCNA
Liquid
PCNA
Recombit fragment corresponding to a region within amino acids 1 and 253 of Human PCNA
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
0.14 mg/mL
PBS with 20% glycerol and 0.025% ProClin 300; pH 7
P12004, P17918, P04961
DNA polymerase delta auxiliary protein, cyclin
Rabbit
IgG
100 μL
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
18538, 25737, 5111
Documents
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