PFKM Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA529336
PA5-29336 targets PFK (muscle) in IF and WB applications and shows reactivity with Human and mouse samples. The PA5-29336 immunogen is recombit fragment corresponding to a region within amino acids 424 and 761 of Human PFK. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 18.104.22.168). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).
|Recombit fragment corresponding to a region within amino acids 424 and 761 of Human PFK|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7|
|GSD7, PFK-1, PFK1, PFKA, PFKX, 6-phosphofructo-1-kinase, 6-phosphofructokinase, muscle type, PFK-A, phosphofructo-1-kinase isozyme A, phosphofructokinase 1, phosphofructokinase, polypeptide X, phosphofructokinase-M, phosphohexokinase|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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