MilliporeSigma anti-PGC-1alpha Clone: 4C1.3,
Mouse Monoclonal Antibody
Manufacturer: MilliporeSigma ST12021SET
Specifically detects PGC-1α Clone: 4C1.3 in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Immunohistochemistry (Paraffin), ImmunoprecipitationPGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors and regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full-length protein is 113kDa and is induced in brown adipose tissue by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full-length gene produces a 270 aa N-terminal splice variant that migrates at ~38kDa and is induced by the same physiological signals that induce expression of the full-length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications that alter its migration and apparent molecular weight.
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|One vial of 100μg antibody in 50mM PBS, see vial for lot-specific concentration. One vial of PGC-1α Positive Control and one vial of NT-PGC-1α Positive Control, supposed as 25μL whole cell extracts in RIPA buffer containing 10mM β-mercaptoethanol and 1% SDS; load 10μL per lane; add sample buffer prior to SDS-PAGE loading.|
|Recognizes the endogenous forms of PGC-1α, which includes the ∽113kDa PGC-1α protein and the ∽38kDa splice variant, in brown adipose tissue, liver, and kidney.|
|Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation|
|a recombinant protein consisting of amino acids 1-120 of mouse PGC-1α|
|Human, Murine, Rat|
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