MilliporeSigma™ anti-phospho-Akt (Ser473), Clone: 6F5, ™

Supplier:  MilliporeSigma™ 051003/DEL

Catalog No. 50-171-458

Description

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including glucose metabolism, cell survival/apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals three isoforms of Akt (Akt1/PKBα, Akt2/PKBβ, and Akt3/PKBγ)exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module to direct Akt to PIP3 at the cell membrane that is necessary for its activation, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of Akt is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blotting

Specifications

• Antigen: phospho-Akt (Ser473)
• Concentration: 1mg/mL
• Host Species: Mouse
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Semi-purified

• Classification: Monoclonal
• Conjugate: Unconjugated
• Quantity: 100 μg
• Research Discipline: Cell Signaling
• Target Species: Human, Mouse, Rat
• Isotype: IgG1 κ