anti-phospho-Akt (Ser473), Clone: 6F5, Millipore™

Manufacturer: EMD Millipore 051003DEL

 View all options for this product

Catalog No. 50-171-458


This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.  View substitute products »


    Antigen phospho-Akt (Ser473)
    Concentration 1mg/mL
    Conjugate Unlabeled
    Cross Reactivity Human,Mouse,Rat
    Format Semi-purified
    Host Species Mouse
    Isotype IgG1 κ
    Quantity 100μg
    Regulatory Status RUO
    Research Discipline Cell Signaling
    Primary or Secondary Primary
    Monoclonal or Polyclonal Monoclonal

    Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including glucose metabolism, cell survival/apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals three isoforms of Akt (Akt1/PKBα, Akt2/PKBβ, and Akt3/PKBγ)exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module to direct Akt to PIP3 at the cell membrane that is necessary for its activation, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of Akt is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

    ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blotting

  • Description & Specifications

Related Products

Product Content Feedback

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit